Abstract

Evaluation of Anthocyanin for its Anti-Angiogenic Potential in Rodent Model

Author(s): Uday P. Kundap* , Sadhana Sathaye**, Rachana D. Sarawade* , Ketan Patel*** and Pankaj D. Jain**

Angiogenesis, the process that leads to the formation of new blood vessels or neovascularization; an important process involved in neovascular diseases and tumor. Angiogenesis inhibitors are designed to prevent the formation of new blood vessels. The main aim of this study is to evaluate Anthocyanin obtained from medicinal plant Ficus.bengalensis for Anti-angiogenesis & to standardize a method for the study of angiogenesis. A variety of animal models have been described to provide more quantitative analysis of in vivo angiogenesis and to characterize pro- and antiangiogenic molecules. However, it is still necessary to establish a quantitative, reproducible and specific method for studies of angiogenesis factors and inhibitors. In-vivo animal models were used for evaluation of antiangiogenesis; in cotton plug Implant model in rats, Cotton plug was implanted in the upper skin of rat in control and experimental group. After 21 days, the cotton plug was analysed for haemoglobin content. It was observed that cotton plug-induced angiogenesis can be suppressed by Anthocyanin, corroborating to the validity of the standardized method. Studies support and accelerate the application of rodent model that predict the anti-angiogenic potential value of compound.

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