Nowadays, the major reason of water pollution is industrial effluent. Chlor-alkali effluent disposed from chlor-alkali paper mill is having very high pH, BOD and COD values as well as organic and inorganic materi-als. Lower concentration of effluents may act as fertilizer but higher concentration has toxic effects. To study impact of Chlor-alkali Effluent (CAE) on plants, seeds of Soyabean (Glycine max), Barbati (Vigna sinensis) and Tomato (Lycopersicum esculentum) were selected for experiment. Parameters selected were height of plant, amount of sugar, non-sugar, protein, ascorbic acid, chlorophyll (a) and chlorophyll (b). Effect of Chlor-alkalieffluent was analyzed at different dilutions viz. 10%, 20%, 40%, 60%, 80%, 100% and control. From thisexperiment, it is clear that with increase of percentage concentration of chlor-alkali effluent, the average length of Glycine max, Vigna sinensisand Lycopersicum esculentumwere decreased. Co-relation was also calculated between concentration and plant length, which showed negative value. So far concerned with amount of sugar, non sugar, protein, ascorbic acid, chlorophyll (a) and chlorophyll (b) in case of these plants, the amount of sugar, non sugar, chlorophyll (a) and chlorophyll (b) remain same at 10% and 20% effluent concentration as compared to control condition and decrease from 40% to 100% effluent concentration as compared to control condition where as the amount of protein and ascorbic acid varies in each species.