Abstract

Phylogenetic Analysis of Noctuoid moths (Lepidoptera: Noctuoidea) by RAPD-PCR Technique

Author(s): Pawan Kumar1* , Manoj Kumar1 & 2 , Shweta Thakur1 & 3 , Rajneesh1 , V. K. Mattu3 & Amit Seth

Noctuoidea is one of the most diverse families within the Lepidoptera. In present study we examined the phylogeny of the Noctuoidea between four species of moths and constructed Phylogenetic tree based on the data from the RAPD technique. Random amplified polymorphic DNA technique has the potential to become a powerful technique for studying genetic relationship and genetic diversity in Lepidopteron. Two primers namely OPA-9 and OPA-13 were used for RAPD analysis. A total of 31 bands were scored with the two decamer primer of them all are monomorphic and percentage of polymorphism is 0%. On the basis of banding pattern of RAPD bands dendrogram constructed with Neighbor joining method which shows Phylogenetic evolution history within four species of Noctuoidea. There is significant difference between branching pattern between the four species which signifies the need of using of RAPD molecular technique for taxonomic classification and to plot the evolutionary relationship between the species.


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